CTech SoloVPE PLUS System

This video from Repligen Process Analytical Solutions discusses the advantages of using Slope Spectroscopy and the SoloVPE system for gene therapy applications. Here are the key points:

• Introduction to Slope Spectroscopy and SoloVPE: Paul Mania explains that slope spectroscopy is a major improvement over traditional UV-Vis methods for gene therapy. The SoloVPE system measures absorbance at different path lengths, so samples don’t need to be diluted [05:45].
• Challenges in Gene Therapy Analytics: Mania discusses the difficulties with current gene therapy analytics, such as high development costs, complex regulations, inconsistent supply, and the absence of fast analytical methods. These problems lead to a high tolerance for error and slow processes [02:50].
• Gene Therapy Applications: The SoloVPE system offers benefits in several gene therapy applications:
  • Culture Density (OD600): It effectively monitors cell growth in E. coli fermentation in real time and without dilution, which reduces errors and saves time [01:29].
  • Antisense Oligonucleotides (ASO): For ASO drug manufacturing, SoloVPE is a simpler, faster, and more reliable method than HPLC, saving 67% of the time and improving precision [14:17].
  • Oligonucleotide Determination: The SoloVPE system is more linear and consistent across a wide range of oligonucleotide concentrations than traditional methods like NanoDrop, which require dilutions for higher concentrations [16:05].
  • Plasmid DNA Purity: SoloVPE allows for precise measurement of plasmid DNA purity, even in complex samples, by removing the need for dilution and improving data reliability [17:01].
• Customer Success and Support: Repligen offers full support for the SoloVPE system, including on-site installation, training, computer system validation documents, and SOP protocols, to ensure easy integration and regulatory compliance [18:06].
• Benefits of SoloVPE System: The SoloVPE system:
  • Reduces variability and potential for failure [19:54].
  • Creates robust processes with quick analytical data (less than one minute) [19:54].
  • Provides real-time data for process optimization [20:00].
  • Is a flexible and scalable platform with high repeatability (+/- 2%) [20:00].
• Global Support Network: Since Repligen acquired C Technologies, a global support network has been created to help customers in Europe, Asia, and North America [19:37].